Guduo Biological Brief ELISA kit and solid phase anti-binding
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A solid phase carrier that has been coated with an antigen or antibody can be stored for 6 months under low temperature and dry conditions, while some incomplete test kits supply only antigen or antibody for coating, and the tester needs to coat it by himself. Solid phase carrier and coating process:
As a adsorbent and container during the ELISA assay, it does not participate in chemical reactions. There are many materials that can be used as carriers in the ELISA kit, and the most commonly used is polystyrene. Polystyrene has a strong ability to adsorb proteins, and the antibody or protein antigen retains its original immunological activity after being adsorbed thereon, and its price is low, so it is widely used. Polystyrene is plastic and can be made in various forms.
There are generally two antigen-antibody reactions in an ELISA, ie, after adding the sample and adding the enzyme conjugate. The completion of the ELISA kit antigen-antibody reaction requires a certain temperature and time. This incubation process is called incubation, which is called incubation and may not be appropriate in ELISA.
The specimens added to the wells in the wells do not have an equal chance of binding to the solid phase, and only the antigen in the solution closest to the well wall is directly in contact with the antibody. This is a process of gradual equilibrium, so it needs to be diffused to reach the end of the reaction. The same is true for the binding of the enzyme-labeled antibody added thereafter to the solid phase antigen. This is why ELISA reactions always require a certain amount of incubation.
Theoretically, the temperatures used for incubation are 43 Â° C, 37 Â° C, room temperature, and 4 Â° C (refrigerator temperature). 37 Â° C is the usual incubation temperature in the laboratory, and is also the appropriate temperature for most antigen-antibody binding. When the ELISA method was established as the reaction kinetics study, the experiment showed that the two antigen-antibody reactions generally reached the peak at 37 Â° C for 1-2 hours.
A casserole is a large, deep dish used both in the oven and as a serving vessel. The word is also used for the food cooked and served in such a vessel, with the cookware itself called a casserole dish or casserole pan.
Casserole cooking is generally done in an oven to bake where heat circulates all around the cooking vessel. Casseroles may be cooked covered or uncovered, while braises are typically covered to prevent evaporation.
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